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pet15b expression vector  (Addgene inc)


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    Structured Review

    Addgene inc pet15b expression vector
    Pet15b Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet15b+expression+vector/SP-Cas9+(Plasmid+%2362731)/pmc12901986-317-11-14
    Average 96 stars, based on 369 article reviews
    pet15b expression vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: The Hairpin Form of r(G 4 C 2 ) exp in c9ALS/FTD is Repeat-associated non-ATG Translated and a Target for Bioactive Small Molecules
    Article Snippet: .. The pet15b expression vector encoding His-tagged hnRNP H1 was obtained from Addgene (ID: 23020)( Chou et al., 1999 ) and transformed into Rosetta 2 (DE3) Escherichia coli cells. ..

    Article Title: Collateral RNA cleavage by CRISPR-Cas13 allows selective cell elimination
    Article Snippet: .. All Cas13 orthologs were cloned into a pET15b expression vector (Addgene #62731). .. LbuCas13a and LshCas13a were a gift from Jennifer Doudna (Addgene plasmids #83482 and #83487).

    Article Title: The Hairpin Form of r(G 4 C 2 ) exp in c9ALS/FTD is Repeat-associated non-ATG Translated and a Target for Bioactive Small Molecules
    Article Snippet: .. 2010 , 53, 3632 N/A BMVC Gift Dr. Ta-Chau Chang (IAMS, Academia Sinica) Critical Commercial Assays In vitro transcription assay Promega Cat# P1320 In vitro translation assay Promega Cat# L4540 TR-FRET assay(StreptavidinXL665) Cisbio Cat# 610SAXLB TR-FRET assay (MAb Anti6His-Tb-cryptate) Cisbio Cat# 61HISTLB Experimental Models: Cell Lines HEK293T ATCC https://www.atcc.org/Products/All/CRL-3216.aspx Oligonucleotides DNA IDT N/A RNA Dharmoncon N/A Recombinant DNA (G 4 C 2 ) 66 Su et al. Neuron 2014 , 83, 1043 N/A (TG 4 C 2 ) 66 This study N/A (G 4 C 2 ) 66 -NoATG-GFP This study N/A (G 4 C 2 ) 66 -NoATG-Nanoluc This study N/A mCherry Addgene Cat#54563 pet15b expression vector encoding His-tagged hnRNP H1 Addgene Cat#2302 Software and Algorithms GraphPad 7.03 Prism https://www.graphpad.com/ Origin 7.5 Originlab https://www.originlab.com/ Topspin 2.1 Bruker https://www.bruker.com/products.html Open in a separate window KEY RESOURCES TABLE Experimental Model Details: Cell culture. ..

    Article Title: Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells
    Article Snippet: .. For recombinant protein production, all Cas13 orthologs were cloned into a pET15b expression vector (Addgene: 62731). .. LbuCas13a and LshCas13a were a gift from Jennifer Doudna (Addgene: 83482 and 83487).

    Plasmid Preparation:

    Article Title: The Hairpin Form of r(G 4 C 2 ) exp in c9ALS/FTD is Repeat-associated non-ATG Translated and a Target for Bioactive Small Molecules
    Article Snippet: .. The pet15b expression vector encoding His-tagged hnRNP H1 was obtained from Addgene (ID: 23020)( Chou et al., 1999 ) and transformed into Rosetta 2 (DE3) Escherichia coli cells. ..

    Article Title: Collateral RNA cleavage by CRISPR-Cas13 allows selective cell elimination
    Article Snippet: .. All Cas13 orthologs were cloned into a pET15b expression vector (Addgene #62731). .. LbuCas13a and LshCas13a were a gift from Jennifer Doudna (Addgene plasmids #83482 and #83487).

    Article Title: The Hairpin Form of r(G 4 C 2 ) exp in c9ALS/FTD is Repeat-associated non-ATG Translated and a Target for Bioactive Small Molecules
    Article Snippet: .. 2010 , 53, 3632 N/A BMVC Gift Dr. Ta-Chau Chang (IAMS, Academia Sinica) Critical Commercial Assays In vitro transcription assay Promega Cat# P1320 In vitro translation assay Promega Cat# L4540 TR-FRET assay(StreptavidinXL665) Cisbio Cat# 610SAXLB TR-FRET assay (MAb Anti6His-Tb-cryptate) Cisbio Cat# 61HISTLB Experimental Models: Cell Lines HEK293T ATCC https://www.atcc.org/Products/All/CRL-3216.aspx Oligonucleotides DNA IDT N/A RNA Dharmoncon N/A Recombinant DNA (G 4 C 2 ) 66 Su et al. Neuron 2014 , 83, 1043 N/A (TG 4 C 2 ) 66 This study N/A (G 4 C 2 ) 66 -NoATG-GFP This study N/A (G 4 C 2 ) 66 -NoATG-Nanoluc This study N/A mCherry Addgene Cat#54563 pet15b expression vector encoding His-tagged hnRNP H1 Addgene Cat#2302 Software and Algorithms GraphPad 7.03 Prism https://www.graphpad.com/ Origin 7.5 Originlab https://www.originlab.com/ Topspin 2.1 Bruker https://www.bruker.com/products.html Open in a separate window KEY RESOURCES TABLE Experimental Model Details: Cell culture. ..

    Article Title: Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells
    Article Snippet: .. For recombinant protein production, all Cas13 orthologs were cloned into a pET15b expression vector (Addgene: 62731). .. LbuCas13a and LshCas13a were a gift from Jennifer Doudna (Addgene: 83482 and 83487).

    Transformation Assay:

    Article Title: The Hairpin Form of r(G 4 C 2 ) exp in c9ALS/FTD is Repeat-associated non-ATG Translated and a Target for Bioactive Small Molecules
    Article Snippet: .. The pet15b expression vector encoding His-tagged hnRNP H1 was obtained from Addgene (ID: 23020)( Chou et al., 1999 ) and transformed into Rosetta 2 (DE3) Escherichia coli cells. ..

    Clone Assay:

    Article Title: Collateral RNA cleavage by CRISPR-Cas13 allows selective cell elimination
    Article Snippet: .. All Cas13 orthologs were cloned into a pET15b expression vector (Addgene #62731). .. LbuCas13a and LshCas13a were a gift from Jennifer Doudna (Addgene plasmids #83482 and #83487).

    Article Title: Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells
    Article Snippet: .. For recombinant protein production, all Cas13 orthologs were cloned into a pET15b expression vector (Addgene: 62731). .. LbuCas13a and LshCas13a were a gift from Jennifer Doudna (Addgene: 83482 and 83487).

    other:

    Article Title: The Hairpin Form of r(G 4 C 2 ) exp in c9ALS/FTD is Repeat-associated non-ATG Translated and a Target for Bioactive Small Molecules
    Article Snippet: To generate the (TG 3 C 2 ) 62 plamsid, gDNA from fibroblasts of a Sca36+ patients was used as a templates in a nested PCR strategy using ThermalAce DNA Polymerase (Invitrogen) or AmpliTaq Gold 360 Polymerase (Thermo Fisher).

    In Vitro:

    Article Title: The Hairpin Form of r(G 4 C 2 ) exp in c9ALS/FTD is Repeat-associated non-ATG Translated and a Target for Bioactive Small Molecules
    Article Snippet: .. 2010 , 53, 3632 N/A BMVC Gift Dr. Ta-Chau Chang (IAMS, Academia Sinica) Critical Commercial Assays In vitro transcription assay Promega Cat# P1320 In vitro translation assay Promega Cat# L4540 TR-FRET assay(StreptavidinXL665) Cisbio Cat# 610SAXLB TR-FRET assay (MAb Anti6His-Tb-cryptate) Cisbio Cat# 61HISTLB Experimental Models: Cell Lines HEK293T ATCC https://www.atcc.org/Products/All/CRL-3216.aspx Oligonucleotides DNA IDT N/A RNA Dharmoncon N/A Recombinant DNA (G 4 C 2 ) 66 Su et al. Neuron 2014 , 83, 1043 N/A (TG 4 C 2 ) 66 This study N/A (G 4 C 2 ) 66 -NoATG-GFP This study N/A (G 4 C 2 ) 66 -NoATG-Nanoluc This study N/A mCherry Addgene Cat#54563 pet15b expression vector encoding His-tagged hnRNP H1 Addgene Cat#2302 Software and Algorithms GraphPad 7.03 Prism https://www.graphpad.com/ Origin 7.5 Originlab https://www.originlab.com/ Topspin 2.1 Bruker https://www.bruker.com/products.html Open in a separate window KEY RESOURCES TABLE Experimental Model Details: Cell culture. ..

    Transcription Assay:

    Article Title: The Hairpin Form of r(G 4 C 2 ) exp in c9ALS/FTD is Repeat-associated non-ATG Translated and a Target for Bioactive Small Molecules
    Article Snippet: .. 2010 , 53, 3632 N/A BMVC Gift Dr. Ta-Chau Chang (IAMS, Academia Sinica) Critical Commercial Assays In vitro transcription assay Promega Cat# P1320 In vitro translation assay Promega Cat# L4540 TR-FRET assay(StreptavidinXL665) Cisbio Cat# 610SAXLB TR-FRET assay (MAb Anti6His-Tb-cryptate) Cisbio Cat# 61HISTLB Experimental Models: Cell Lines HEK293T ATCC https://www.atcc.org/Products/All/CRL-3216.aspx Oligonucleotides DNA IDT N/A RNA Dharmoncon N/A Recombinant DNA (G 4 C 2 ) 66 Su et al. Neuron 2014 , 83, 1043 N/A (TG 4 C 2 ) 66 This study N/A (G 4 C 2 ) 66 -NoATG-GFP This study N/A (G 4 C 2 ) 66 -NoATG-Nanoluc This study N/A mCherry Addgene Cat#54563 pet15b expression vector encoding His-tagged hnRNP H1 Addgene Cat#2302 Software and Algorithms GraphPad 7.03 Prism https://www.graphpad.com/ Origin 7.5 Originlab https://www.originlab.com/ Topspin 2.1 Bruker https://www.bruker.com/products.html Open in a separate window KEY RESOURCES TABLE Experimental Model Details: Cell culture. ..

    Recombinant:

    Article Title: The Hairpin Form of r(G 4 C 2 ) exp in c9ALS/FTD is Repeat-associated non-ATG Translated and a Target for Bioactive Small Molecules
    Article Snippet: .. 2010 , 53, 3632 N/A BMVC Gift Dr. Ta-Chau Chang (IAMS, Academia Sinica) Critical Commercial Assays In vitro transcription assay Promega Cat# P1320 In vitro translation assay Promega Cat# L4540 TR-FRET assay(StreptavidinXL665) Cisbio Cat# 610SAXLB TR-FRET assay (MAb Anti6His-Tb-cryptate) Cisbio Cat# 61HISTLB Experimental Models: Cell Lines HEK293T ATCC https://www.atcc.org/Products/All/CRL-3216.aspx Oligonucleotides DNA IDT N/A RNA Dharmoncon N/A Recombinant DNA (G 4 C 2 ) 66 Su et al. Neuron 2014 , 83, 1043 N/A (TG 4 C 2 ) 66 This study N/A (G 4 C 2 ) 66 -NoATG-GFP This study N/A (G 4 C 2 ) 66 -NoATG-Nanoluc This study N/A mCherry Addgene Cat#54563 pet15b expression vector encoding His-tagged hnRNP H1 Addgene Cat#2302 Software and Algorithms GraphPad 7.03 Prism https://www.graphpad.com/ Origin 7.5 Originlab https://www.originlab.com/ Topspin 2.1 Bruker https://www.bruker.com/products.html Open in a separate window KEY RESOURCES TABLE Experimental Model Details: Cell culture. ..

    Article Title: Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells
    Article Snippet: .. For recombinant protein production, all Cas13 orthologs were cloned into a pET15b expression vector (Addgene: 62731). .. LbuCas13a and LshCas13a were a gift from Jennifer Doudna (Addgene: 83482 and 83487).

    Software:

    Article Title: The Hairpin Form of r(G 4 C 2 ) exp in c9ALS/FTD is Repeat-associated non-ATG Translated and a Target for Bioactive Small Molecules
    Article Snippet: .. 2010 , 53, 3632 N/A BMVC Gift Dr. Ta-Chau Chang (IAMS, Academia Sinica) Critical Commercial Assays In vitro transcription assay Promega Cat# P1320 In vitro translation assay Promega Cat# L4540 TR-FRET assay(StreptavidinXL665) Cisbio Cat# 610SAXLB TR-FRET assay (MAb Anti6His-Tb-cryptate) Cisbio Cat# 61HISTLB Experimental Models: Cell Lines HEK293T ATCC https://www.atcc.org/Products/All/CRL-3216.aspx Oligonucleotides DNA IDT N/A RNA Dharmoncon N/A Recombinant DNA (G 4 C 2 ) 66 Su et al. Neuron 2014 , 83, 1043 N/A (TG 4 C 2 ) 66 This study N/A (G 4 C 2 ) 66 -NoATG-GFP This study N/A (G 4 C 2 ) 66 -NoATG-Nanoluc This study N/A mCherry Addgene Cat#54563 pet15b expression vector encoding His-tagged hnRNP H1 Addgene Cat#2302 Software and Algorithms GraphPad 7.03 Prism https://www.graphpad.com/ Origin 7.5 Originlab https://www.originlab.com/ Topspin 2.1 Bruker https://www.bruker.com/products.html Open in a separate window KEY RESOURCES TABLE Experimental Model Details: Cell culture. ..

    Cell Culture:

    Article Title: The Hairpin Form of r(G 4 C 2 ) exp in c9ALS/FTD is Repeat-associated non-ATG Translated and a Target for Bioactive Small Molecules
    Article Snippet: .. 2010 , 53, 3632 N/A BMVC Gift Dr. Ta-Chau Chang (IAMS, Academia Sinica) Critical Commercial Assays In vitro transcription assay Promega Cat# P1320 In vitro translation assay Promega Cat# L4540 TR-FRET assay(StreptavidinXL665) Cisbio Cat# 610SAXLB TR-FRET assay (MAb Anti6His-Tb-cryptate) Cisbio Cat# 61HISTLB Experimental Models: Cell Lines HEK293T ATCC https://www.atcc.org/Products/All/CRL-3216.aspx Oligonucleotides DNA IDT N/A RNA Dharmoncon N/A Recombinant DNA (G 4 C 2 ) 66 Su et al. Neuron 2014 , 83, 1043 N/A (TG 4 C 2 ) 66 This study N/A (G 4 C 2 ) 66 -NoATG-GFP This study N/A (G 4 C 2 ) 66 -NoATG-Nanoluc This study N/A mCherry Addgene Cat#54563 pet15b expression vector encoding His-tagged hnRNP H1 Addgene Cat#2302 Software and Algorithms GraphPad 7.03 Prism https://www.graphpad.com/ Origin 7.5 Originlab https://www.originlab.com/ Topspin 2.1 Bruker https://www.bruker.com/products.html Open in a separate window KEY RESOURCES TABLE Experimental Model Details: Cell culture. ..



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